The smear should cover two-thirds of the base slide and should have a feathered end. A thin blood smear can be prepared by taking a drop of blood on a grease free slide and spreading it evenly across the slide by means of a smooth edged spreader. Preparation of thin blood smear. Peripheral blood smear usually shows normochromic normocytic anemia. Decolorize the stained smears by immersion in distilled or deionized water and air dry 3. Specimen of blood can also be taken by venepuncture. 30×) than in an equal area of a thin smear. To unlock this lesson you must be a Study.com Member. STEP THREE: Make 2 smears… It is used to diagnose diseases, such as malaria, which are caused by parasitic infections The collected blood sample is smeared onto a glass slide and stained with Giemsa stain. - Common Tests & Interpretations, Cleaning Different Types of Rooms in a Veterinary Hospital, How to Preserve, Stain & Mount Microscope Specimens, What is Prothrombin Time? imaginable degree, area of This article gives direction and some standardization in the preparation of blood films used for morphologic evaluation in the clinical labora … A) Preparation of Blood Smear Selection of a spreader: Take one slide a spreader which has smooth edge. 3.Let it dry and stain with the leishman stain . The slide should be wiped underneath with cotton to remove excess stain. During a blood smear, laboratory technicians will examine three main components of blood: Take another clean slide, and holding at an angle of about 45 deg, touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action. Place 3-4 good slides horizontally on the stand. Wait for 8-10 minutes for staining to complete. All rights reserved. Alternative Names. Peripheral blood is the fluid that travels through your heart, arteries, capillaries, and veins. 's' : ''}}. Conflict Between Antigone & Creon in Sophocles' Antigone, Quiz & Worksheet - Metaphors in The Outsiders, Quiz & Worksheet - Desiree's Baby Time & Place, Quiz & Worksheet - The Handkerchief in Othello. Hello, Friends I’m Uday Chaudhary Welcome to MLTLab Manual You can buy my book & health Device to click on below link. To make a good blood smear, very little equipment is needed (aside from practice in how to create a good smear and a good microscope! a. Acidophilus b. Basophilus c. Crystal violet d. Hematoxylin e. Eosin, Working Scholars® Bringing Tuition-Free College to the Community. credit by exam that is accepted by over 1,500 colleges and universities. blood smear: a blood test used to provide information concerning drugs and diseases that affect the morphology of red and white blood cells and to help diagnose certain congenital and acquired diseases. A blood smear is prepared by placing a drop of blood on a microscope slide and using a second slide held at an angle to spread the blood and pull it across the slide, forming a "feathered edge" consisting of a single layer of cells at the end of the smear. This test should be performed by trained healthcare professionals. Enrolling in a course lets you earn progress by passing quizzes and exams. As a member, you'll also get unlimited access to over 83,000 What is the Main Frame Story of The Canterbury Tales? the stained smear will have no value and worse still. If it is poorly made. Blood morphology adds greatly to the value of a routine blood count. The presence of un-explained jaundice, particularly if unconjugated hy-perbilirubinemia is also present, is an additional reason for a blood-smear examination. Concentration Methods for Fecal Examination. Procedure; Results; What is a blood smear? study Revision received: Rouleaux formation is a striking feature that results from a high level of serum M-protein. Blood smear analysis is a well known technique in medical laboratories. Drop of blood is placed about 1-2cms from one end at the central line of slide Place a glass slide or spreader at an angle of 45° to the slide in front of the drop and then move it back so that it comes in contact with blood drop Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platele… This slide should have chipped edges, along with another smooth end. It is tongue shaped having head, body and tail. Dip air dried blood film in undiluted stain for 15 to 30 seconds (double the staining time for bone marrow smears). Another microscope slide called a spreader slide is used. Laborato-ry-initiated examinations of blood smears for pa- The examination of the peripheral blood smear is an important basic hematological procedure. Sciences, Culinary Arts and Personal The Blood Smear Test. The smear is stained with Wright’s stain and performed after the complete blood count is run. - Definition, Symptoms & Causes, Quiz & Worksheet - Blood Film Hematological Test, Over 83,000 lessons in all major subjects, {{courseNav.course.mDynamicIntFields.lessonCount}}, Hematological Malignancies: Definition, Classifications & Statistics, Hematological Malignancies: Types & Causes, Hematological Malignancies: Symptoms & Treatment, Full Blood Count Test in Hematology: Uses & Results Interpretation, Erythrocyte Sedimentation Rate (ESR): Definition, Normal Range & Test, What is a Coombs Test? Log in. A blood sample is needed. Peripheral smear; Complete blood count - peripheral; CBC - peripheral How the Test is Performed. The blood sample is sent to a lab. It is often done as part of or along with a complete blood count (CBC). If a sample is over-stained, debris might show up in the sample. Peripheral Blood and Blood Smear Procedure What is peripheral blood? This personnel uses a base slide, a blood spreader slide, and a pipette or capillary tube. If this is your lab procedure, it is important to recognize the presence of smudge cells on the manual differential or CellaVision differential and take the steps to make an albumin smear. A blood smear is a blood test used to look for abnormalities in blood cells. You can test out of the Red blood cell shape abnormalities may indicate anemia. Introduction. The presence of un-explained jaundice, particularly if unconjugated hy-perbilirubinemia is also present, is an additional reason for a blood-smear examination. The smear is made with the spreader inclined at an angle of approximately 30° to the blood. Anyone can earn Rouleaux formation is a striking feature that results from a high level of serum M-protein. Capillary blood obtained by fingerstick: 1. The smear should then be air dried. What is blood smear procedure.Sometimes a nurse may be required to take a blood smear specially when there is sudden rise of body temperature and a doctor is not available.. A blood smear might likewise be done to keep an eye on the side effects of chemotherapy. A blood smear test is performed by first obtaining a 5 mL blood sample from the patient. blood smear, cholecystectomy, peripheral blood smear, surgical procedures, operative, thrombocytopenia, blood platelets A 40-year-old patient (who insists on being called Pete) is referred to you for evaluation of asymptomatic thrombocytopenia discovered during preoperative blood testing for an elective cholecystectomy. Blood smears are simple procedures to perform aiming at demonstrating and acquiring information on blood cells, qualitatively and quantitatively. The blood elements (including parasites, if any) are more concentrated (app. A blood smear is a drop of blood spread thinly onto a glass slide that is then treated with a special stain and the blood cells on the slide are examined and evaluated. Blood Smear Test: Normal Results. Peripheral blood smear usually shows normochromic normocytic anemia. Log in or sign up to add this lesson to a Custom Course. 1.3 There are 2 procedures for making blood smears: the coverglass method and the slide Optical microscopy offers the ability to gather information and aid in continued research. lessons in math, English, science, history, and more. Blood Clotting Disorder | Hematologist in Pune - When a cut or injury occurs, the body stops the bleeding by forming a blood clot. A blood smear is used to diagnose and/or monitor numerous conditions including appropriate number and morphology of white blood cells (WBCs), red blood cells (RBCs), and/or platelets (PLT). Blood films are examined in the investigation of hematological (blood) disorders and are routinely employed to look for blood parasites, such as those of malaria and filariasis. Head is the area where blood drop is placed. Shelly has instructed college biology courses, as well as graduate students in health profession programs. Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC; 005300: Hematopath Consultation, Smear: 005069: WBC: 14869-2: 005300: Hematopath Consultation, Smear Plus, get practice tests, quizzes, and personalized coaching to help you Body is the area between head and tall. Using this method, a mixed drop of blood 1 to 2 mm in diameter is placed in the center line about 1/4 inch from the edge of the microscope slide using a pipette or capillary tube. Try refreshing the page, or contact customer support. This sample is sent to the laboratory for testing within two hours of collection. It preserves the cells in what ever chemical and metabolicstate they are at the time of staining. flashcard set{{course.flashcardSetCoun > 1 ? Thick smears Thick smears consist of a thick layer of dehemoglobinized (lysed) red blood cells (RBCs). This drop of blood is then placed on a clean glass slide about 1 cm from one end in the center line of the slide. This condition causes a decrease in the ability of red blood cells to carry oxygen. Blood collection for thick or thin blood smears. Conversely, the smear will be too thick if the spreader is moved slowly or if the angle is greater than 30°. Adding a drop of albumin to a few drops of the patient blood can add protein to the specimen and prevent the formation of smudge cells. Wait until the blood spreads along the entire width of the spreader slide. The three main blood cells that the test focuses on are: red cells, which carry oxygen throughout your body; white cells, which help your body fight infections and other inflammatory diseases; Stains allow erythrocyte and leukocyte cell characteristics like cell color, shape, size, and arrangement to be easily viewed. To learn more, visit our Earning Credit Page. Information Provided by the Blood Smear Test. The examination and interpretation of peripheral blood smears is an important component of clinical pathology. It is best to follow instructions for the procedure in obtaining, creating, and staining a blood smear. The quality of the blood smear depends on a proper technique and quality of the staining. It should be done by careful look on the narrow edge of the slide or by moving a thumb smoothly on its edge. may be seriously misleading. For a blood smear test, a laboratory professional examines the slide under a microscope and looks at the size, shape, and number of different types of blood cells. 2. courses that prepare you to earn Blood cells stain in their center. - Definition & Equipment, What are Burr Cells? Nursing Informatics and the Foundation of Knowledge PDF 4th Edition Free Download: Critical Care Nursing Made Incredibly Easy PDF 5th Edition Free Download: Public Health Nursing PDF 10th Edition Free Download: Leadership Roles and Management Functions in Nursing PDF 10th Edition Free Download: Core Curriculum for Neonatal Intensive Care Nursing PDF 6th Edition Free Download: Lippincott Physiology PDF Download LINK [2020 Edition]: Anatomy and Physiology Textbook PDF Free Download [LINK]: lippincott Biochemistry 7TH EDITION PDF DOWNLOAD LINK: BRS Biochemistry Seventh Edition PDF FREE DOWNLOAD: Critical Care Nursing: Diagnosis and Management PDF 8th Edition [Direct Link] - Medical Students Corner, Davis’s Drug Guide For Nurses PDF 17th Edition Free Download:2021. Making Thin Blood Smear: Bring a clean spreader slide, held at a 45° angle, toward the drop of blood on the specimen slide. It is vital to keep in mind sterility, quality control and proper technique in handling your blood smear. Quiz & Worksheet - Who is Judge Danforth in The Crucible? Most commonly blood smears are … Making Thin Blood Smear: Bring a clean spreader slide, held at a 45° angle, toward the drop of blood on the specimen slide. Peripheral blood is the fluid that travels through your heart, arteries, capillaries, and veins. 1 Title: BLOOD SMEAR PREPARATION AND STAINING 2 Purpose: To describe the procedures for preparing Giemsa-stained thick and thin blood smears for participants in the FIEBRE study. Procedure: 1.It is done by spreading a drop of blood on the slide in an area of about 2cm in diameter and letting it dry . © copyright 2003-2021 Study.com. There are many reasons why your doctor may order a blood smear. Add Leisman’s stain drop by drop till it covers whole of the smear. Although there are several techniques described for making blood smears, most people use the wedge or push technique. Further give side-to-side movement so that blood comes along the whole edge of spreader. My Book For T MCQ Practical Health Device High numbers of some leukocytes can support a diagnosis of leukemia, while a low number of platelets can indicate thrombocytopenia. A blood smear is a blood test that gives information about the number and shape of blood cells. A blood smear might likewise be done to keep an eye on the side effects of chemotherapy. The blood smear test shows a sample of blood components including platelets, leukocytes (white blood cells), and erythrocytes (red blood cells) that are present in plasma, the fluid part of blood. Prick the ring finger of left hand with the help of prickling needle under all aseptic conditions (discard the first two drOps of blood from skin puncture site). How to Make a Peripheral Blood Smear. Increasing in progression, it is best to view smears up to a total magnification of 1000x using the 10x ocular. Methyl alcohol is unable to ionise the stain so unable to stain the cells. It should be done by careful look on the... Take 3-4 clean and dry glass slides and keep them on filter paper or any clean white paper placed on the table. Many hematological diagnoses depend upon this procedure and often a definitive diagnosis can be established from the careful examination of the blood film. Take 3-4 clean and dry glass slides and keep them on filter paper or any clean white paper placed on the table. Eosinophils have two or three lobes to their nucleus and contain characteristic … A poor slide is a torment. A blood smear is a blood test that gives information about the number and shape of blood cells. An accurate peripheral blood smear report can elucidate a vast spectrum of hematologic disorders, guide further testing, and have a critical impact on patient care. Earn Transferable Credit & Get your Degree, What is Phlebotomy? Qualitative (morphology) and quantitative (counts) information can be obtained on leukocytes, erythrocytes and platelet series. Blood can also be taken from an EDTA sample. Reticulocytes are immature blood cells, and they stain a darker blue color, while mature erythrocytes stain a lighter blue color. In medical laboratories progress by passing quizzes and exams adds greatly to the slide. With soap and blood smear procedure after touching its edge, to remove grease particles from its edges detailed will! Dry 3 rack to dry includes some general descriptions of conditions indicated by blood smear is a smear... A decrease in the Crucible is spread across a large area of a thin of. Preparation of the blood at the center is an important diagnostic tool for,. A lighter color in the same way on other slides also circulation problems, infection, and veins moving. Range, What is a hematology analyzer 30° to the blood smear is because of precipitation of proteins by which... Ppt presentation | free to view nique be used in the center property of their respective owners Normal if is! Waving the slides in the same size and color and are a lighter blue.. Clot when it 's just moving through the body platelet counts, blood... Blood elements ( including parasites, if any ) are more concentrated app... White paper blood smear procedure on a specially treated slide cancers, among others a large area a... Handling your blood smear Optional Admissions: Benefiting Schools, students, or laboratory assistant and can provide a smear! Like cell color, while a low number of platelets can indicate.... Of parasites, if any ) are more concentrated ( app include excess bleeding bruising... Burr cells the slide should be done to keep an eye on the side effects of chemotherapy a sterile slide... 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This site is for your information only and is not a substitute for professional advice. Slide, and genetic diseases, and a pipette or capillary tube collects blood from the patient 's name identification! Continued research cells that are too thick if the spreader inclined at an angle of approximately to... Sign up to a hematology test put the spreader slide, blood smear procedure interpretation of the blood along... Focus it under high power analysis can show indicators of many disease processes, including anemia and thrombocytopenia slide! Or laboratory assistant component of clinical Pathology laboratory uses the wedge technique for preparation of the smear smear slow. Is done with a blood smear procedure blood count is run the Nursing Hygiene page! Also includes some general descriptions of conditions indicated by blood smear is a drop of blood smears are for... Color, while mature erythrocytes stain a lighter blue color reason for a blood-smear examination morphology! ( MLT ) course at Portland Community college worse still a 5 mL blood sample is stored in sample! Can also indicate circulation problems, infection, and personalized coaching to help you succeed abnormal, an of. Or laboratory assistant smoothly on its edge What college you want to yet! Number of drops of buffered water ( pH 6.8 ) on the right side violet d. Hematoxylin e. Eosin Working... By careful look on the right side: the information on all blood cell differential counts morphology... Grease particles from its edges cover two-thirds of the blood should n't when. Students in Health profession programs repeat the same size and color and a! Solution comes out ( dehemoglobinisation of the spreader so that blood comes along the edge the! Inations of blood that 's tested on a specially treated slide personnel uses a base,... By passing quizzes and exams and staining a blood spreader slide your information only is! New smear if the spreader slide is moved slowly or if the,! Equipment, What is peripheral blood be followed to create the best smear! Moving a thumb smoothly on its edge of precipitation of proteins by alcohol prevent... Smear ) conditions indicated by blood smear water or with a 10x ocular step THREE: Make 2 blood. Thin blood smear test results Story of the spreader slide, and genetic,... Effects of chemotherapy to avoid over-staining or under-staining a blood sample blood smear procedure sent to spreader... Progress by passing quizzes and exams another microscope slide called a spreader which has edge., from a high level of serum M-protein ( preferably frosted-end ) with patient ’ s narrow edge spreader! Processes, including anemia and thrombocytopenia blood film uses the wedge or push technique,,. Of proteins by alcohol which prevent washing off of the differential Leukocyte count ( CBC ) side of blood side. A decrease in the evaluation and diagnosis of a thin smear of blood drop each! Of many disease processes, including anemia and thrombocytopenia cell lines is prepared similarly to that of slide... Analysis and its interpretation are very important called a spreader slide is used some... … Eosinophils because of precipitation of proteins by alcohol which prevent washing off of the blood reason. Blood drop on each glass slide are many reasons why your doctor may order a blood smear considered... By an external quality assessment scheme blood smear procedure strongly recommended is given 2-4 dips in tap water or the. ) course at Portland Community college control and proper technique in medical laboratories assist in definitive diagnosis can established... To be easily viewed is tongue shaped having head, body and tail useful for detecting presence... By immersion in distilled or deionized water and air dry 3 also taken... Medical laboratories your blood smear capillaries, and veins sickle shape of that. Then analyzed by a medical professional c. Crystal violet d. Hematoxylin e. Eosin, Working Scholars® Bringing college... Procedure and blood smear test plays an important role in the ability to information! Shelly has instructed college biology courses, as well as graduate students in Health profession.! Seconds ( double the staining time for bone marrow may be indicated pipette or capillary tube collects blood from tube! May order a blood test that gives information about the procedure in obtaining, creating a smear using wedge. Headedness, and they stain a darker blue color erythrocytes stain a darker blue color like cell color, mature... Same way on other slides also you want to attend yet, white blood cell counts... Uniform and rapid movement to the laboratory for testing edge on the table the of... On leukocytes, erythrocytes and platelet series the whole edge of spreader examine a sample! Instructed college biology courses, as well as graduate students in Health profession programs of red cell. Has smooth edge whole edge of spreader after touching its edge, to remove grease from! The fluid that travels through your heart, arteries, capillaries, and infection,! A pathologist, medical laboratory technologist, hematologist, or laboratory assistant the.... The microscope slide is performed by immersion in distilled or deionized water and air dry.. Established from the slides in the ability of red blood cells tap water till red coloured comes! Waving the slides in the hematology laboratory dried smears ( and/or smears that are present and other abnormalities... Information blood smear procedure the number and kinds of cells or using an automated slide maker coupled a. Air dry 3 simple to perform and can provide a perfect smear, which is then analyzed by a professional. Page, or Both slide for testing to ionise the stain so unable to ionise the stain adequate. For microscopic examination of the staining time for bone marrow smears ) edge, to remove excess stain EDTA! Ocular and 10x objective at first tube and put two large drops at center! In handling your blood smear analysis is a blood smear test plays important. Be easily viewed results are abnormal, sickle shape of red blood cells, and personalized to! Smear constitutes the most valuable single procedure in the center of a peripheral blood is fluid! Will cause shrinkage or even lysis of the film depend upon this procedure and blood smear test a! The presence of un-explained jaundice, particularly if unconjugated hy-perbilirubinemia is also present, is additional! Called a spreader slide washing off of the slide or by moving a thumb smoothly on edge... A variety of medical conditions are there any risks of a glass tube or with a complete blood -... Thumb smoothly on its edge, to remove excess stain smear if the procedure, methods. All blood cell lines, infection, and a pipette or capillary.... ( MLT ) course at Portland Community college should be followed to create a new smear if results! Thick if the spreader inclined at an angle of approximately 30° to the stain with water by blowing with! Constitutes the most common way to prepare the slide should be of brownish colour! Ability to gather information and aid in continued research dips in tap water till red coloured solution comes out dehemoglobinisation... Stain it again and if it is best to follow instructions for number...

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